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• Original Article •
Evaluation of myocardial fibrosis in miniature pig model of COPD by the 3.0 T magnetic resonance LGE techniques: An experimental study
LIU Zhi  GUO Dandan  LI Chunping  LI Rui 

Cite this article as: Liu Z, Guo DD, Li CP, et al. Evaluation of myocardial fibrosis in miniature pig model of COPD by the 3.0 T magnetic resonance LGE techniques: An experimental study[J]. Chin J Magn Reson Imaging, 2021, 12(8): 49-54. DOI:10.12015/issn.1674-8034.2021.08.010.


[Abstract] Objective To explore the feasibility of 3.0 T cardiovascular magnetic resonance (CMR) late gadolinium enhancement (LGE) techniques in assessing early left ventricular (LV) myocardial fibrosis in miniature pig COPD model, so as to provide imaging basis for detecting early myocardial injury in COPD. Materials andMethods Twenty healthy Bama miniature pigs were divided into COPD model group (16 pigs, COPD model was prepared by protease) and normal control group (4 pigs). Before modeling and at different stages of modeling (4 weeks, 8 weeks and 20 weeks), multimodal CMR scanning was performed. The differences of conventional cardiac parameters among the groups were compared, and the incidence, distribution and types of LV myocardial LGE were analyzed.Results The COPD model of 14 miniature pigs in the model group was successfully constructed. There was no significant difference in baseline data between the model group and the control group in the early and middle stage of modeling (4 weeks and 8 weeks) (P＞ 0.05); however, in the late stage of modeling (20 weeks), respiratory rate and heart rate were significantly increased (P=0.003 and 0.002 respectively); body weight was decreased (P=0.007). There was no significant difference in heart function parameters between groups in the early and middle stages of modeling (P＞0.05). The right ventricular anterior wall (RVAW) (all P＜0.001) was thickened, while the right ventricular end diastolic diameter (RVDd) (P＜0.001) and left ventricular posterior wall (LVPW) (P=0.018) was only thicker than before modeling, left ventricular end diastolic diameter (LVDD) (P=0.05) and left ventricular end diastolic volume (LVEDV) (P=0.005) were decreased than before modeling. The right ventricular ejection fraction (RVEF) was lower than that before modeling and in the control group (P＜0.001 and 0.001); the left ventricular ejection fraction (LVEF) had no statistical difference (P＞0.05). LGE was found in 23 myocardial segments (23/85) of 5 minipigs in the model group after modeling, which was significantly different from that in the control group and before modeling (all P＜0.01). LGE was mainly located in the basal and middle slices of left ventricle, and the distribution was statistically significant (P＜0.05), and the most common type of LGE was intramural type.Conclusions In the early stage of COPD, decreased RVEF, but still in the normal range could be observed. Myocardial fibrosis occurred in a small part of LV myocardium, accompanied with normal LVEF. LGE technology based on CMR could provide imaging basis for early intervention, diagnosis and treatment planning and prognosis evaluation of COPD patients.
[Keywords] chronic obstructive pulmonary disease；cardiovascular magnetic resonance；miniature pigs；late gadolinium enhancement；myocardial injury；fibrosis

LIU Zhi1, 2   GUO Dandan2   LI Chunping2   LI Rui2*  

1 Chongqing Hospital of Traditional Chinese Medicine, Chongqing 400021, China

2 Affiliated Hospital of North Sichuan Medical College, and Sichuan Key Laboratory of Medical Imaging, Nanchong 637000, China

Li R, E-mail: ddtwg_nsmc@163.om

Conflicts of interest   None.

ACKNOWLEDGMENTS This work was part of National Natural Science Foundation of China (No. 81801674); Basic Research on Application of Science and Technology Department of Sichuan Province (No. 2021YJ0242); Scientific Research Project of Sichuan Provincial Health Commission (No. 19PJ201).
Received  2021-03-23
Accepted  2021-05-24
DOI: 10.12015/issn.1674-8034.2021.08.010
Cite this article as: Liu Z, Guo DD, Li CP, et al. Evaluation of myocardial fibrosis in miniature pig model of COPD by the 3.0 T magnetic resonance LGE techniques: An experimental study[J]. Chin J Magn Reson Imaging, 2021, 12(8): 49-54. DOI:10.12015/issn.1674-8034.2021.08.010.

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